使用目的

Brucella Antibody Rapid Test is a lateral flow immunoassay intended for the qualitative detection of Brucella Antibody (BRU Ab) in serum/plasma sample.

内容

仕様

オペレーション

結果

病理学的紹介

犬ブルセラ症はブルセラ菌によって引き起こされる人獣共通感染症である。主に犬のブルセラ症や生殖障害を引き起こすほか、椎間板炎、骨髄炎、髄膜脳炎、ぶどう膜炎などを引き起こすこともある。

Adult dogs infected with Brucella rarely show severe clinical symptoms, or may only exhibit lymphadenitis. They may also show systemic symptoms after a latent period of 2 weeks to as long as 6 months. Pregnant bitches often miscarry between 40 to 60 days of pregnancy. Within the 1 to 6 weeks before miscarriage, infected dogs generally do not have high body temperature, but the vulva and vaginal mucosa are swollen, and there may be light brown or greyish-green discharge from the vagina.

Miscarried fetuses often exhibit partial tissue autolysis, subcutaneous edema, congestion, and subcutaneous hemorrhage in the abdominal region. In early pregnancy (10 to 20 days after mating), embryos may die and be absorbed by the mother’s body. Miscarried bitches may develop uteritis and subsequent infertility after repeated mating. Male dogs may develop orchitis, epididymitis, scrotal swelling, scrotal dermatitis, and sperm abnormalities. In addition to reproductive system symptoms, infected dogs may also develop arthritis, tenosynovitis, and occasionally limping. Some infected dogs may also develop uveitis.

検出方法

When pregnant bitches have miscarriages, bitches are infertile, or male dogs have orchitis or epididymitis, this disease should be suspected. A comprehensive diagnosis can be made on the basis of epidemiology, clinical symptoms, bacteriological examinations, and serological reactions.

妊娠した雌犬の流産が多く、雌犬の不妊が繰り返される場合や、雄犬が睾丸炎、精巣上体炎、陰嚢の腫脹、繁殖能力の低下などを起こす犬群では、この病気を疑う必要があります。そのような雄犬の精液塗抹標本には、腫れた異常細胞が多数認められることがあります。

After dogs are infected with Brucella, bacteremia can persist for months to years. Therefore, taking blood for bacterial culture is the best method for diagnosis. Aseptically collected blood samples are inoculated into nutrient broth and cultured under aerobic conditions for 3 to 5 days. Then these samples are subcultured onto solid culture media for identification.

Brucella in dogs grows relatively slowly, taking 48 to 96 hours to form visible colonies. Alternatively, samples can be taken from aborted fetal membranes, fetal stomach contents, or diseased liver, spleen, and lymph node tissues. They are made into smears, stained (using Macchiavello or modified Ziehl-Neelsen methods) and examined under a microscope. Red-colored bacteria can be seen under the microscope.  In this way, the disease is diagnosed.

Conventional testing methods require specific laboratory environments and trained personnel. But the ITGEN Canine Brucella Antibody Rapid Test Strip is easy to use, unaffected by environmental factors or personnel, and is suitable for initial screening of antibodies against canine brucellosis.

治療／予防計画

予防のためには、以下のような包括的な対策を講じる必要がある：

犬の個体群（特に繁殖個体群）の血清学的検査を定期的に行い、必要であれば血液培養を行う。これらの検査は年に2回行うのがベストで、陽性の犬は厳重に隔離する。繁殖目的では陰性の犬だけを飼育する。

できるだけ自家繁殖を奨励する。新規に入手した犬は1ヶ月間隔離して観察し、検疫を受け、健康状態が良好であることが確認された後にのみ、個体群に導入すべきである。

雄の繁殖犬は、繁殖に参加する前に検疫を受け、健康であることを確認しなければならない。

Dog kennels and exercise areas should be regularly disinfected. The areas, pens or other equipment contaminated by miscarriage materials should be thoroughly disinfected.

経済的に価値のない病気の犬は安楽死させることができる。価値のある病気の犬は隔離して治療することができるが、獣医学的な衛生対策を厳格に実施しなければならない。