In the ever-evolving field of pet healthcare, early detection and diagnosis are key to effective treatment and recovery. One of the most convenient tools in veterinary diagnostics is the use of test kits, which allow for the rapid identification of various diseases using small samples. These diagnostic test kits which were developed and produced by Antigenne can use a range of sample types including secretions, feces, whole blood/serum/plasma and cloaca. For example, Canine ジステンパー Virus Antigen Rapid Test is a valuable diagnostic tool for early detection of the セル遅延変動 Ag by using secretions as examples.

1. Sample Types Used in Diagnostic Test kits

Diagnostic test kits are designed to detect specific diseases in animals quickly and efficiently, using minimally invasive samples. The most commonly used sample types include:

• Secretions:These include nasal, eye, and genital secretions, and are commonly used to detect respiratory infections, viral or bacterial diseases, and other localized infections.
• Whole blood/serum/plasma:Used in many diagnostic test kits for detecting pathogens like viruses, bacteria, and parasites, or measuring antibodies and hormones.
• Feces: Often used to test for gastrointestinal parasites and infections.

Among these, secretion-based test kits are particularly effective for diagnosing respiratory and eye infections, where sample collection is both easy and non-invasive for the animal. Now, let’s look at the diseases that can be detected through secretions.

2. Diseases Detectable with Secretion-Based Test kits

Test kits that utilize secretions as the sample type can be applied to diagnose a range of diseases, especially in pets like dogs and cats. Some of the key diseases that can be detected through nasal, ocular, or genital secretions include:

• イヌ ジステンパーVirus (CDV): A highly contagious viral disease affecting dogs, especially puppies and unvaccinated adults.
• Feline Herpesvirus (FHV): A common cause of upper respiratory infections in cats, often presenting as nasal discharge and eye infections.
• Canine Parainfluenza Virus (CPIV): Another respiratory infection causing symptoms such as nasal discharge and coughing.
• Bordetella bronchiseptica: A bacterial infection in dogs and cats, which can also cause respiratory problems, often resulting in nasal discharge.

Today, we will take Canine Distemper Virus Antigen Rapid Test (CDV Ag) developed and produced by Antigenne testing that use secretions as a sample type as a example, taking a deeper look at the process of using these kits. Let’s first understand the disease, its causes, and its symptoms.

3. CDV (Canine Distemper Virus) and Its Symptoms

犬ジステンパー・ウイルス（CDV）は、主に家畜の犬や、キツネ、オオカミ、アライグマなどの野生動物に感染するウイルスによって引き起こされる、重篤でしばしば致死的な病気です。ウイルスは空気中や、鼻や目の分泌液など感染した体液に直接触れることで広がります。子犬やワクチン未接種の犬は、この病気に最もかかりやすい。

Causes of CDV:

• The virus is mainly transmitted through airborne exposure (e.g., from coughing or sneezing by infected dogs).
• Contact with contaminated surfaces, food bowls, or direct contact with an infected animal.

Symptoms of CDV:

• Early signs include fever, nasal discharge, eye discharge, coughing, and lethargy.
• As the disease progresses, dogs may develop gastrointestinal symptoms like vomiting and diarrhea.
• In more severe cases, the virus affects the central nervous system, causing seizures, tremors, or paralysis.

How to test for distemper in dogs? 犬ジステンパー検査 Kit is a valuable diagnostic tool for early detection of the virus, enabling quick intervention and increasing the chances of recovery. Next, we will outline how to use the CDV Ag Rapid Test.

4. セル遅延変動 Ag test kit instructions

Using the CDV Ag test キット involves three simple steps. These test kits are designed to be user-friendly and require minimal handling to provide accurate results.

ステップ1：使用前のキット内容の確認

• 製品内容を確認し、室温下（15～30℃）でテストする。
• キットの内容には、検査装置、綿棒、緩衝液入り抽出チューブ、添付文書、ワークステーションが含まれる。

Step 2: Test Procedure

• バッファーの入った抽出チューブの封を開ける。
• 抽出チューブをワークステーションにセットする。
• Use the swab to collect secretions specimen from canine/feline eye, nasal fluid, or saliva.Then, put the swab into the buffer.
• 綿棒を10回以上回転させる。
• バッファーチューブのキャップを閉める。
• Take the test device out of the aluminum foil bag, and place it on a clean and flat table. Add three drops (about 90 μl) of specimen(mixed sample) vertically into the  specimen well (S) of the test device.

ステップ3：検査結果の解釈

• 5～10分後に結果を読む。結果は15分後に無効になる。
• 陽性（+）：T線の強弱にかかわらず、C線とT線の両方が存在すること。
• マイナス（-）：クリアCラインのみ表示。
• 無効：無効：T ラインの出現に関係なく、C 領域に色の付いたラインは現れない。

5.警告、注意および安全性

• The test device is used for canine only.
• 結果は湿度や温度に影響される可能性がある。
• 開封前に、試験の入ったフォイルパウチが破損していないことを確認してください。パウチが開封されたら、直ちに試験を行ってください。
• テスト部品を再利用しないでください。
• 使用期限後は使用しないでください。
• 異なるロット番号の製品コンポーネントを混合しないでください。

6.検査キットの限界

一方 CDV Ag test キット は、オフィス内での迅速な検査に非常に有用であるが、注意すべき限界もある：

しかし CDV Ag Rapid Test is very accurate in detecting Canine Distemper Virus Antigen, a low incidence of false results may be occurred. Other clinically or laboratory tests might be required if questionable results are obtained. As other diagnostic tests, a definitive clinical diagnosis should not be based on the result of a single test, but should be diagnosed by the veterinarian after all clinical and laboratory findings have been evaluated.

結論

Secretion-based diagnostic test kits, such as the Canine Distemper Virus Antigen Rapid Test (CDV Ag), are powerful tools in veterinary medicine, offering a quick and reliable at home distemper test method for detecting diseases. However, understanding the limitations of these kits is equally important, and complementary tests may be necessary for a thorough diagnosis. By adhering to these guidelines, pet owners and veterinarians can ensure timely intervention and improve the prognosis for affected animals.

アンティジェンヌ is committed to helping pet health management through innovative technology, and the rapid detection kit for Canine Distemper Virus Antigen is an outstanding product of the company in the field of rapid diagnosis of canine intestinal diseases. For more information on purchasing the Canine Distemper Virus Antigen Rapid Test (CDV Ag), visit our website or contact our customer service team. Ensure your pet’s health with Antigenne’s advanced diagnostic solutions.