FELINE S IgE TEST KIT

FELINE S IgE TEST KIT

The Vamber FELINE S IgE TEST KIT is designed to determine the levels of Specific IgE (S IgE) against different allergens in feline serum or plasma.

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Hangzhou Antigenne Technology Co., Ltd

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♥High accuracy & sensitivity

♥Simple operation, easy to use, stable

♥Rapid: Get results in 5-10 minutes

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Product Details

INTENDED USE

FELINE S IgE TEST KIT is designed to determine the levels of Specific IgE (S IgE) against different allergens in feline serum or plasma.

 

KIT CONTENTS

Contents Quantity
Solid Array Unit with a protective cap and a desiccant packaged in an aluminum foil bag 2
Solution Unit 2
Substrate 2
Manual Interpretation package, containing GuideLocator, and Color Scale 1
Result Card 2
Instruction Manual 1

 

TEST PROCEDURE 

Test Procedure  Download

 

TEST RESULTS

Guide of Manual Interpretation Download 

 

DESIGN AND PRINCIPLE

For one sample testing, one Solid Array Unit, one Solution Unit and one Substrate should be used together.

 

The Solid Array Unit, which contains an array composed of location markers and allergens on a membrane, and a protective cap are packaged in one aluminum foil bag with a desiccant.

 

The Solution Unit contains all the necessary reagents for forming enzyme linked complex of antibody-antigen reaction that are deposited separately in the different compartments of a plastic cartridge and sealed with a protective aluminum foil.

 

The Substrate is deposited in a small substrate bottle.

 

Briefly, open the Solution Unit and deposit the sample in the compartment 1 of theSolution Unit and mix well. Take the Solid Array Unit from the aluminum foil bag and pull off the protective cap.

 

Then insert the Solid Array Unit into the compartment 1 and have it absorb the solution

in the compartment 1 for a few minutes. After the absorption, the pink dye will disappear from the membrane in the window, which indicates successful specific antibody-antigen reaction finished

 

Then transfer the Solid Array Unit to the remaining compartments at timed intervals step by step. The bound feline lgE antibodies on the protein array will be labeled with enzyme through biotin-streptavidin reaction. The anti-feline lgE-biotin conjugate is deposited in compartment 3, and the streptavidin-enzyme conjugate is deposited in compartment 5.

 

For a satisfactory result, wash steps are introduced. In the compartment 2, the unbound feline lgE antibodies in the sample will be removed. In compartment 4 , the unbound anti-feline lgE-biotin conjugate will be removed. In the compartment 6, 7 and 8, the unbound streptavidin-enzyme conjugate will be adequately removed.

 

At the end, pipette Substrate in the substrate bottle, and add it on the membrane at the window center to develop purple-blue spots if there were enzyme bound there.

 

To confirm the validation of the performance, purple-blue color of the location markers on the membrane should be visible.

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