Canine Relaxin (Canine Relaxin) is a peptide hormone produced by luteal cells during canine pregnancy that plays an important role in pregnancy maintenance, uterine and mammary gland development. As research has progressed, assays for relaxin dog test have become increasingly diverse. In this article, we will introduce several major methods and principles for detecting Canine Relaxin, as well as the differences between them.
Immunoassay
Immunoassay is one of the commonly used methods for the relaxin dog pregnancy test, and its principle is based on the specific binding between antigen and antibody. An antibody specific for Canine Relaxin is prepared and binds to Canine Relaxin in the sample to be tested, forming an antigen-antibody complex, and then the concentration of Canine Relaxin is indirectly determined by measuring the amount of the complex.
Enzyme-linked immunosorbent assay (ELISA):
ELISA is a highly sensitive and specific immunoassay. It uses enzyme-labelled antibody to bind to Canine Relaxin to form an enzyme-labelled antigen-antibody complex, and then the enzyme catalyzes the colour development of the substrate to quantify the concentration of Canine Relaxin by measuring the shade of colour.
Radioimmunoassay (RIA):
RIA uses radioisotope-labelled antibodies to bind to Canine Relaxin to form a radioactive antigen-antibody complex, and then quantifies the concentration of Canine Relaxin by measuring the intensity of radioactivity of the complex. Although RIA has a high degree of sensitivity and specificity, it is complicated to perform and carries certain safety risks because it involves radioactive substances.
The SaberVet Canine Relaxin Rapid Blood Test offered by Antigenne is a simple, convenient and accurate method to aid in the diagnosis of early relaxin pregnancy hormone in dogs. Below is a detailed description of the test:
Principle of the relaxin pregnancy test for dogs
The test is based on elevated levels of early progesterone in the blood of canines during early pregnancy (approximately days 25 to 30).
Early pregnancy can be quickly diagnosed by fluorescent immunochromatography or a coloured latex test.
Features of dog relaxin test
Animal species: exclusively for canines.
Sample type: Plasma or serum can be used as sample.
Sample volume: A sample volume of 75μL is required.
Test range: The test range is 0.5-50 ng/mL.
Reaction time: The entire assay takes approximately 15 minutes.
Storage conditions: Store at 4-30°C. Shelf life is up to 24 months.
Reference Values of relaxin test for dogs
During non-pregnancy, the reference value for Canine Relaxin in canine blood is usually less than 3 ng/mL.
During pregnancy, Canine Relaxin levels increase to 3 ng/mL and above.
Summary of canine relaxin test
The Canine Relaxin Rapid Blood Test provides veterinarians with an easy and quick way to do relaxin test for pregnancy in dogs.By measuring plasma or serum levels of Canine Relaxin, veterinarians can obtain an accurate diagnosis in the early stages of canine pregnancy. The assay has a wide detection range, short reaction time and long shelf life, providing veterinarians with great convenience in their clinical work.
Mass Spectrometry
Mass spectrometry is a mass spectrometer-based assay that analyses the composition and structure of a substance by measuring the ion mass-to-charge ratio (m/z). In the detection of Canine Relaxin, the molecule of Canine Relaxin can be ionised into ions by a mass spectrometer and its mass-to-charge ratio can be measured to determine its molecular weight and structure. Mass spectrometry is highly sensitive and accurate, but requires expensive equipment and specialised operating skills.
Bioactivity Assay
Bioactivity assay is a method of indirectly determining the concentration of Canine Relaxin by observing its action on organisms or cells. For example, the relaxing effect of Canine Relaxin on uterine smooth muscle can be used to determine its bioactivity. This method is simple to operate, but the results are affected by a variety of factors, such as the interference of other biologically active substances in the sample, changes in experimental conditions, and so on.
Differences between methods of relaxin test for dogs
- Sensitivity and specificity: Immunoassay and mass spectrometry usually have high sensitivity and specificity and can accurately detect the concentration of Canine Relaxin. On the other hand, bioactivity assays are affected by many factors, and their sensitivity and specificity are relatively low.
- Operation complexity: Immunoassay and bioactivity assay are relatively simple to operate and suitable for routine laboratory use. Mass spectrometry, on the other hand, requires expensive equipment and professional operating skills, and has a higher degree of operating complexity.
- Cost: Immunoassay and bioactivity assay are relatively low cost and suitable for large-scale testing. Mass spectrometry, on the other hand, requires expensive equipment and maintenance costs, and is more costly.
In conclusion, various methods for the detection of Canine Relaxin have their own advantages and disadvantages, and the appropriate method should be selected for the detection according to the experimental conditions and requirements.
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